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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in several tumors, which results in chromosome instability, and thus might add to tumorigenesis. This protein was found to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a vital part in selling cell-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, happen to be explained. Two more splice variants were documented but in-depth nucleotide sequence details is just not nevertheless accessible.
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Blocking peptides are peptides that bind particularly towards the goal antibody and block antibody binding. These peptide normally has the epitope regarded by the antibody.
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This tutorial outlines even further probable optimization of mobile and immunoassay parameters to be sure the absolute best final results are...
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This manual outlines further doable optimization of mobile and immunoassay parameters to make sure the absolute best results are...
Antibodies certain to the blocking peptide not bind for the epitope to the target protein. By comparing the staining through the blocked antibody versus the antibody on your own, one can see which staining is unique.
Concentrate on Information Cyclin E belongs for the hugely conserved cyclin spouse and children, whose members are characterized by a remarkable periodicity in protein abundance with the cell cycle. Cyclins operate as regulators of CDK kinases. Unique cyclins show unique expression and degradation patterns which contribute for the temporal coordination of each mitotic function. Cyclin E kinds a complex with and capabilities to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates with the G1-S stage boundary and it is degraded as cells development by means of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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